Proteina | Protein-Protein Interactions

PPIs deliver unique cellular insights

In the vital process of human cells, up to 650,000 protein-protein interactions (PPIs) control biological activities. Cells with aberrant metabolism, including cancer cells, are dependent on specific PPIs. Conventional diagnostic tests merely characterize proteins expressed from the gene—they do not measure the interactions between these proteins.

Measuring disease-specific PPIs triggered by abnormal cell activities provides crucial information that genomic methods do not provide, filling a critical gap to advance research.

A new approach to measuring protein characteristics

Proteina has developed a novel method for measuring protein characteristics—including PPIs, protein expression levels, and post-translational modifications—quickly and accurately.

Extracts from tissue, blood and cells contain protein complexes.

An antibody is used to target a specific protein within the protein complex on the Proteina chip.

A fluorescently labelled antibody that targets other proteins within the protein complex binds to the protein complex.

The Proteina imaging platform collects the signal and quantifies the protein complexes as point images.

Focusing on key protein molecular events

Proteina’s unique approach to PPI profiling unlocks potential therapeutic targets by focusing on four molecular events:

Pre-formed PPI complex

Proteins form a large signaling complex as a functional module. This reflects the current situation of cell signaling in a sample.

Protein expression/PTM

Amounts and post-translational modification (PTM) status are the basis of PPI. Proteina quantifies these by preserving the delicate conformation of target proteins.

PPI reservoir

Proteins with a vacant binding pocket are potential candidates for PPI. Proteina’s proprietary technology directly measures the strength of potential PPI.

Molecular
platform

A protein complex is a signaling hub triggering many biological processes. We quantify the size and amount of these molecular hubs with single-molecule imaging.

Proteina is shifting the paradigm from genetic tests to PPI-based disease diagnosis and treatment.

Learn about our technology platform

Revealing the power of the PPI universe